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KMID : 0613820020120020200
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Journal of Life Science
2002 Volume.12 No. 2 p.200 ~ p.207
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Isolation and Antifungal Activity of Bacillus ehimensis YJ-37 as Antagonistic against Vegetables Damping-off Fungi
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Joo Gil-Jae
Kim Jin-Ho
Kang Sang-Jae
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Abstract
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This study was carried out to isolate of antagonistic bacterium against Pythium ultimum and Rhizoctonia solani AG-4, causal pathogens of vegetables damping-off. Total of 600 strains were isolated from soil and plant roots. The isolates were screened for antagonism against Pythium ultimum and Rhizoctonia solani AG-4. One strain, named YJ-37, was sellected for detained study among those microoganisms screened. It was identified as Bacillus ehimensis based on morphological and physiological characterisitics according to the Bergey’s mannual of systematic bacteriology, Sherlock system of Microbial ID Inc. and 16S rDNA sequences methods. Furthermore Bacillus ehimensis YJ-37 showed antifungal activities against Alternaria altrata, Collectotrichum gloeosporioides, Didymella bryoniae, Fusarium moniliforme, Fusarium oxysporum, F. oxysporum cucumerinum, F. oxysporum niveum, Gloeosporium sp., Glomerella sp., G. cingulata, G. lagenaria, Penicillium digitatum, P. italicum, Phytophthora capsici, Sclerotinia sclerotiorum, and Stemprhylium solani.
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KEYWORD
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Antagonistic, Bacillus ehimensis, Pythium ultimum, Rhizoctonia solani AG-4
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